Image Processing

All-in-one multifunctional optical microscope with a single holographic measurement

[+] Author Affiliations
Cheng Liu, Yoon Sung Bae, Wenzhong Yang, Dug Young Kim

Gwangju Institute of Science and Technology, Department of Information and Communications, Buk-gu, Gwangju, South Korea 500-712

Opt. Eng. 47(8), 087001 (August 13, 2008). doi:10.1117/1.2968708
History: Received March 27, 2008; Revised May 23, 2008; Accepted May 23, 2008; Published August 13, 2008
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An all-purpose multifunctional optical microscope based on digital holographic microscopy (DHM) is proposed, which enables DHM to be practically suitable for bioimaging applications. With our simple suggested digital signal-processing scheme, we have demonstrated that various optical images, including pure amplitude, pure phase, dark-field, phase-contrast, and differential interference contrast images, can be obtained from a single DHM measurement. Because these images obtained with our method are essentially the same as those obtainable with conventional optical imaging instruments, biologists can easily analyze them with their previous knowledge and experience.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Cheng Liu ; Yoon Sung Bae ; Wenzhong Yang and Dug Young Kim
"All-in-one multifunctional optical microscope with a single holographic measurement", Opt. Eng. 47(8), 087001 (August 13, 2008). ; http://dx.doi.org/10.1117/1.2968708


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