Design of an optical system consisting of a special telecentric lens for side-scattering measurement on individual cells

Lu Zhang; Hong Zhao; Weibin Dong; Nan Cao; Pengfei Zhang

doi:10.1117/1.3425657

1 May 2010 Design of an optical system consisting of a special telecentric lens for side-scattering measurement on individual cells

Lu Zhang, Hong Zhao, Weibin Dong, Nan Cao, Pengfei Zhang

Author Affiliations +

Optical Engineering, Vol. 49, Issue 5, 053001 (May 2010). https://doi.org/10.1117/1.3425657

Abstract

The alignment and collection of side-scattered light in scattering measurement on individual cells, based on hydraulic focusing technology, is always time-consuming and troublesome. Even small errors result in a poor SNR. This paper presents an optical system including a specially designed telecentric lens to focus stray light and scattering signals. The lens has a compact structure, small distortion and aberration, and large depth of focus. Thus scattering signals can be extracted easily and effectively, even when the spatial filters are out of focus or tilted with respect to the optical axis by human error. Also, the stray light in the side direction is analyzed, and the results show good performance of this special telecentric lens. Scattering results on 4.91- and 16.32-µm polystyrene beads verify that this optical system can be well applied for side-scattering signal detection.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Lu Zhang, Hong Zhao, Weibin Dong, Nan Cao, and Pengfei Zhang "Design of an optical system consisting of a special telecentric lens for side-scattering measurement on individual cells," Optical Engineering 49(5), 053001 (1 May 2010). https://doi.org/10.1117/1.3425657

Published: 1 May 2010

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