Imaging Systems

Vertical differential interference contrast

[+] Author Affiliations
Michael Warber, Tobias Haist, Malte Hasler, Wolfgang Osten

University of Stuttgart, Institut für Technische Optik, Stuttgart Center of Photonic Engineering (SCOPE)Pfaffenwaldring 9, 70569 StuttgartGermany

Opt. Eng. 51(1), 013204 (Feb 06, 2012). doi:10.1117/1.OE.51.1.013204
History: Received September 27, 2011; Revised November 22, 2011; Accepted November 29, 2011
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Abstract.  We propose a new phase contrast filtering technique based on a combination of a focused and a defocused point-spread-function. This way, an axial shear is introduced in the imaging system. Compared to conventional differential interference contrast, an isotropic behavior is achieved. The lateral resolution is improved compared to conventional defocusing. Furthermore, the digital combination of multiple images leads to strongly enhanced visualization of small structures. We show simulated results as well as experimental results using a spatial-light modulator-based microscope.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Michael Warber ; Tobias Haist ; Malte Hasler and Wolfgang Osten
"Vertical differential interference contrast", Opt. Eng. 51(1), 013204 (Feb 06, 2012). ; http://dx.doi.org/10.1117/1.OE.51.1.013204


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