Holography

Dual-wavelength digital holographic imaging with phase background subtraction

[+] Author Affiliations
Alexander Khmaladze

University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, Michigan 48109

Rebecca L. Matz

University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, Michigan 48109

Joshua Jasensky

University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, Michigan 48109

Emily Seeley

University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, Michigan 48109

Mark M. Banaszak Holl

University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, Michigan 48109

Zhan Chen

University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, Michigan 48109

Opt. Eng. 51(5), 055801 (May 18, 2012). doi:10.1117/1.OE.51.5.055801
History: Received December 15, 2011; Revised March 15, 2012; Accepted March 20, 2012
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Abstract.  Three-dimensional digital holographic microscopic phase imaging of objects that are thicker than the wavelength of the imaging light is ambiguous and results in phase wrapping. In recent years, several unwrapping methods that employed two or more wavelengths were introduced. These methods compare the phase information obtained from each of the wavelengths and extend the range of unambiguous height measurements. A straightforward dual-wavelength phase imaging method is presented which allows for a flexible tradeoff between the maximum height of the sample and the amount of noise the method can tolerate. For highly accurate phase measurements, phase unwrapping of objects with heights higher than the beat (synthetic) wavelength (i.e. the product of the original two wavelengths divided by their difference), can be achieved. Consequently, three-dimensional measurements of a wide variety of biological systems and microstructures become technically feasible. Additionally, an effective method of removing phase background curvature based on slowly varying polynomial fitting is proposed. This method allows accurate volume measurements of several small objects with the same image frame.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Alexander Khmaladze ; Rebecca L. Matz ; Joshua Jasensky ; Emily Seeley ; Mark M. Banaszak Holl, et al.
"Dual-wavelength digital holographic imaging with phase background subtraction", Opt. Eng. 51(5), 055801 (May 18, 2012). ; http://dx.doi.org/10.1117/1.OE.51.5.055801


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