True color blood flow imaging using a high-speed laser photography system

Chien-Sheng Liu; Cheng-Hsien Lin; Yung-Nien Sun; Chung-Liang Ho; Chung-Chi Hsu

doi:10.1117/1.OE.51.10.103201

1 October 2012 True color blood flow imaging using a high-speed laser photography system

Chien-Sheng Liu, Cheng-Hsien Lin, Yung-Nien Sun, Chung-Liang Ho, Chung-Chi Hsu

Author Affiliations +

Optical Engineering, Vol. 51, Issue 10, 103201 (October 2012). https://doi.org/10.1117/1.OE.51.10.103201

Abstract

Physiological changes in the retinal vasculature are commonly indicative of such disorders as diabetic retinopathy, glaucoma, and age-related macular degeneration. Thus, various methods have been developed for noninvasive clinical evaluation of ocular hemodynamics. However, to the best of our knowledge, current ophthalmic instruments do not provide a true color blood flow imaging capability. Accordingly, we propose a new method for the true color imaging of blood flow using a high-speed pulsed laser photography system. In the proposed approach, monochromatic images of the blood flow are acquired using a system of three cameras and three color lasers (red, green, and blue). A high-quality true color image of the blood flow is obtained by assembling the monochromatic images by means of image realignment and color calibration processes. The effectiveness of the proposed approach is demonstrated by imaging the flow of mouse blood within a microfluidic channel device. The experimental results confirm the proposed system provides a high-quality true color blood flow imaging capability, and therefore has potential for noninvasive clinical evaluation of ocular hemodynamics.

Citation Download Citation

Chien-Sheng Liu, Cheng-Hsien Lin, Yung-Nien Sun, Chung-Liang Ho, and Chung-Chi Hsu "True color blood flow imaging using a high-speed laser photography system," Optical Engineering 51(10), 103201 (1 October 2012). https://doi.org/10.1117/1.OE.51.10.103201

Published: 1 October 2012

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