Imaging Components, Systems, and Processing

Time-gated stimulated emission depletion nanoscopy

[+] Author Affiliations
Yifan Wang

Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, Hangzhou 310027, China

Cuifang Kuang

Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, Hangzhou 310027, China

Zhaotai Gu

Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, Hangzhou 310027, China

Yingke Xu

Zhejiang University, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Hangzhou 310027, China

Shuai Li

Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, Hangzhou 310027, China

Xiang Hao

Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, Hangzhou 310027, China

Xu Liu

Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, Hangzhou 310027, China

Opt. Eng. 52(9), 093107 (Sep 25, 2013). doi:10.1117/1.OE.52.9.093107
History: Received June 18, 2013; Revised August 19, 2013; Accepted August 21, 2013
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Abstract.  We have designed and built a time-gated continuous wave stimulated emission depletion (CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line time-gating detection was performed with the help of time-correlated single-photon counting technique. Experimental results showed that before time-gating, the resolution of our system was about 75 nm with a depletion beam (592 nm) power of 200 mW. By using the off-line time-gating detection, the resolution was further improved to 38 nm. Biological samples were also used to test the performance of our time-gated CW-STED, and a resolution of 70 nm was achieved with a depletion beam (592 nm) power of 85 mW. Detailed principles of time-gated CW-STED were discussed in the text. The time-gated STED provides a better resolution with finite laser power.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Yifan Wang ; Cuifang Kuang ; Zhaotai Gu ; Yingke Xu ; Shuai Li, et al.
"Time-gated stimulated emission depletion nanoscopy", Opt. Eng. 52(9), 093107 (Sep 25, 2013). ; http://dx.doi.org/10.1117/1.OE.52.9.093107


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