Imaging Components, Systems, and Processing

Object field expansion in spatial light modulator-based phase contrast microscopy

[+] Author Affiliations
Malte Hasler, Janek Stahl, Tobias Haist, Wolfgang Osten

Universität Stuttgart, Institut für Technische Optik, Pfaffenwaldring 9, 70569 Stuttgart, Germany

Opt. Eng. 54(4), 043107 (Apr 20, 2015). doi:10.1117/1.OE.54.4.043107
History: Received February 6, 2015; Accepted March 23, 2015
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Abstract.  In order to expand the field of view in spatial light modulator-based phase contrast microscopy, we employ a two-image recording method with digital postprocessing. Using this approach, we suppress the superimposed unwanted diffraction orders on the image sensor, thus eliminating the necessity to assure a strict separation of the diffraction orders. These methods allow for a significantly smaller microscope setup and an increased effective spatial bandwidth product. We apply this method for a number of phase contrast methods to show its capabilities and limitations. Mainly, the signal-to-noise ratio is decreased by η/2. Furthermore, we employ a simple field-dependent aberration correction scheme to improve the image quality.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Malte Hasler ; Janek Stahl ; Tobias Haist and Wolfgang Osten
"Object field expansion in spatial light modulator-based phase contrast microscopy", Opt. Eng. 54(4), 043107 (Apr 20, 2015). ; http://dx.doi.org/10.1117/1.OE.54.4.043107


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