Instrumentation, Techniques, and Measurement

Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy

[+] Author Affiliations
Shouyu Wang

Jiangnan University, School of Science, Department of Optoelectronic Information Science and Engineering, No. 1800, Lihu, Wuxi, Jiangsu 214122, China

Keding Yan

Xi’an Technological University, School of Electronic Information Engineering, No. 2 Xuefu Middle Road, Xi’an, Shaanxi 710032, China

Yanke Shan, Mingfei Xu, Fei Liu

Nanjing Agricultural University, College of Veterinary Medicine, Single Molecule Nanometry Laboratory, No. 1 Weigang Road, Nanjing, Jiangsu 210095, China

Liang Xue

Shanghai University of Electric Power, College of Electronics and Information Engineering, No. 2103 Pingliang Road, Shanghai 200090, China

Opt. Eng. 54(12), 124105 (Dec 30, 2015). doi:10.1117/1.OE.54.12.124105
History: Received October 13, 2015; Accepted December 1, 2015
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Abstract.  Erythrocyte morphology is an important factor in disease diagnosis, however, traditional setups as microscopes and cytometers cannot provide enough quantitative information of cellular morphology for in-depth statistics and analysis. In order to capture variations of erythrocytes affected by metal ions, quantitative interferometric microscopy (QIM) is applied to monitor their morphology changes. Combined with phase retrieval and cell recognition, erythrocyte phase images, as well as phase area and volume, can be accurately and automatically obtained. The research proves that QIM is an effective tool in cellular observation and measurement.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Shouyu Wang ; Keding Yan ; Yanke Shan ; Mingfei Xu ; Fei Liu, et al.
"Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy", Opt. Eng. 54(12), 124105 (Dec 30, 2015). ; http://dx.doi.org/10.1117/1.OE.54.12.124105


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