Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography

Yanlu Lv; Jiulou Zhang; Dong Zhang; Lin Zhang; Jianwen Luo

doi:10.1117/1.OE.55.12.123113

23 December 2016 Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography

Yanlu Lv, Jiulou Zhang, Dong Zhang, Lin Zhang, Jianwen Luo

Author Affiliations +

Optical Engineering, Vol. 55, Issue 12, 123113 (December 2016). https://doi.org/10.1117/1.OE.55.12.123113

Abstract

A simple, compact, and inexpensive tunable volume holographic imaging spectrometer is presented to acquire narrowband multispectral fluorescence measurements. It is constructed with three simple achromatic lenses, a transmission volume hologram, and a detector array. Experiments are carried out to evaluate the performance of this spectrometer. The experimental results indicate that the tunable spectrometer can operate at a wavelength range from 400 to 700 nm. Additionally, this spectrometer can provide a lateral field-of-view greater than 33 mm and a spatial resolution of 1.0 mm under narrow band illumination (full-width-half-maximum bandwidth 20 nm). Multispectral fluorescence molecular tomography experiments are conducted with this spectrometer to test the applicability of narrowband multispectral macroscopy.

Citation Download Citation

Yanlu Lv, Jiulou Zhang, Dong Zhang, Lin Zhang, and Jianwen Luo "Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography," Optical Engineering 55(12), 123113 (23 December 2016). https://doi.org/10.1117/1.OE.55.12.123113

Received: 20 July 2016; Accepted: 30 November 2016; Published: 23 December 2016

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