Imaging Components, Systems, and Processing

Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography

[+] Author Affiliations
Yanlu Lv, Jiulou Zhang, Dong Zhang, Lin Zhang

Tsinghua University, School of Medicine, Department of Biomedical Engineering, Haidian District, Beijing 100084, China

Jianwen Luo

Tsinghua University, School of Medicine, Department of Biomedical Engineering, Haidian District, Beijing 100084, China

Tsinghua University, Center for Biomedical Imaging Research, Haidian District, Beijing 100084, China

Opt. Eng. 55(12), 123113 (Dec 23, 2016). doi:10.1117/1.OE.55.12.123113
History: Received July 20, 2016; Accepted November 30, 2016
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Abstract.  A simple, compact, and inexpensive tunable volume holographic imaging spectrometer is presented to acquire narrowband multispectral fluorescence measurements. It is constructed with three simple achromatic lenses, a transmission volume hologram, and a detector array. Experiments are carried out to evaluate the performance of this spectrometer. The experimental results indicate that the tunable spectrometer can operate at a wavelength range from 400 to 700 nm. Additionally, this spectrometer can provide a lateral field-of-view greater than 33 mm and a spatial resolution of 1.0 mm under narrow band illumination (full-width-half-maximum bandwidth 20 nm). Multispectral fluorescence molecular tomography experiments are conducted with this spectrometer to test the applicability of narrowband multispectral macroscopy.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Yanlu Lv ; Jiulou Zhang ; Dong Zhang ; Lin Zhang and Jianwen Luo
"Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography", Opt. Eng. 55(12), 123113 (Dec 23, 2016). ; http://dx.doi.org/10.1117/1.OE.55.12.123113


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