We propose and demonstrate an application of microfiber knot resonator (MKR) in the generation of a stable and uniform single-wavelength erbium-doped fiber laser (EDFL). An MKR was fabricated using a microfiber a few micrometers in diameter. By embedding the MKR to the ring cavity of the EDFL, a laser with a wavelength of 1558.818 nm and a 3-dB linewidth of 0.0149 nm is demonstrated. The side mode suppression ratio of the laser is about 30 dB, and the maximum power fluctuation is about 0.85 dB. The results demonstrate that the MKR can be employed as a high-performance comb filter to realize a stable and uniform fiber laser.