Instrumentation, Techniques, and Measurement

Super-resolution quantitative phase-contrast imaging by microsphere-based digital holographic microscopy

[+] Author Affiliations
Qiaowen Lin

Beijing University of Technology, College of Applied Sciences, Beijing, China

Shanxi Datong University, School of Physics and Electronics Science, Datong, Shanxi, China

Dayong Wang, Yunxin Wang, Sha Guo, Spozmai Panezai, Liting Ouyang, Lu Rong, Jie Zhao

Beijing University of Technology, College of Applied Sciences, Beijing, China

Opt. Eng. 56(3), 034116 (Mar 25, 2017). doi:10.1117/1.OE.56.3.034116
History: Received December 22, 2016; Accepted February 28, 2017
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Abstract.  A super-resolution quantitative phase-contrast imaging method using high refractive index microsphere is developed to overcome the diffraction limit of optical field, which is produced by the object in the digital holographic microscopy. A microsphere placed on the surface of the object can collect the underlying near-field information, which appears as the evanescent waves and transforms them into propagating waves. Due to the spherical symmetry provided by the microsphere, the super-resolution of the imaging system can be realized in all directions at the same time with one-shot recording. The experiments are carried out for a cosine grating with the line width of 255 nm as the object, which confirms that the lateral resolution can be less than λ/2. Meanwhile, the quantitative phase-contrast image is experimentally obtained. The reconstructed complex field distribution provides the great flexibility with the digital processing for the microscope imaging, such as the ability of refocusing and numerical reconstruction.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Qiaowen Lin ; Dayong Wang ; Yunxin Wang ; Sha Guo ; Spozmai Panezai, et al.
"Super-resolution quantitative phase-contrast imaging by microsphere-based digital holographic microscopy", Opt. Eng. 56(3), 034116 (Mar 25, 2017). ; http://dx.doi.org/10.1117/1.OE.56.3.034116


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