Materials, Photonic Devices, and Sensors

Fluorescent sensing with Fresnel microlenses for optofluidic systems

[+] Author Affiliations
Anna Siudzińska, Andrzej Miszczuk, Jacek Marczak, Katarzyna Komorowska

Wrocław Research Centre EIT+, Department of Nanotechnology, Wrocław, Poland

Opt. Eng. 56(5), 057106 (May 22, 2017). doi:10.1117/1.OE.56.5.057106
History: Received March 15, 2017; Accepted April 28, 2017
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Abstract.  The concept of fluorescent sensing in a microchannel equipped with focusing light Fresnel lenses has been demonstrated. The concept employs a line or array of Fresnel lenses generating a line or array of focused light spots within a microfluidic channel, to increase the sensitivity of fluorescent signal detection in the system. We have presented efficient methods of master mold fabrication based on the lithography method and focused ion beam milling. The flexible microchannel was fabricated by an imprint process with new thiolene-epoxy resin with a good ability to replicate even submicron-size features. For final imprinted lenses, the measured background to peak signal level shows more than nine times the increase in brightness at the center of the focal spot for the green part of the spectrum (532 nm). The effectiveness of the microlenses in fluorescent-marked Escherichia coli bacteria was confirmed in a basic fluoroscope experiment, showing the increase of the sensitivity of the detection by the order of magnitude.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Anna Siudzińska ; Andrzej Miszczuk ; Jacek Marczak and Katarzyna Komorowska
"Fluorescent sensing with Fresnel microlenses for optofluidic systems", Opt. Eng. 56(5), 057106 (May 22, 2017). ; http://dx.doi.org/10.1117/1.OE.56.5.057106


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