Special Section on Interferometry

Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells

[+] Author Affiliations
Jianglei Di, Yu Song, Teli Xi, Jiwei Zhang, Ying Li, Chaojie Ma, Kaiqiang Wang, Jianlin Zhao

Northwestern Polytechnical University, MOE Key Laboratory of Material Physics and Chemistry under Extraordinary Conditions, Shaanxi Key Laboratory of Optical Information Technology, School of Science, Xi’an, China

Opt. Eng. 56(11), 111712 (Jul 11, 2017). doi:10.1117/1.OE.56.11.111712
History: Received April 15, 2017; Accepted June 15, 2017
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Abstract.  Biological cells are usually transparent with a small refractive index gradient. Digital holographic interferometry can be used in the measurement of biological cells. We propose a dual-wavelength common-path digital holographic microscopy for the quantitative phase imaging of biological cells. In the proposed configuration, a parallel glass plate is inserted in the light path to create the lateral shearing, and two lasers with different wavelengths are used as the light source to form the dual-wavelength composite digital hologram. The information of biological cells for different wavelengths is separated and extracted in the Fourier domain of the hologram, and then combined to a shorter wavelength in the measurement process. This method could improve the system’s temporal stability and reduce speckle noises simultaneously. Mouse osteoblastic cells and peony pollens are measured to show the feasibility of this method.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Jianglei Di ; Yu Song ; Teli Xi ; Jiwei Zhang ; Ying Li, et al.
"Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells", Opt. Eng. 56(11), 111712 (Jul 11, 2017). ; http://dx.doi.org/10.1117/1.OE.56.11.111712


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