Pathological leucocyte segmentation algorithm based on hyperspectral imaging technique

Yana Guan; Qingli Li; Hongying Liu; Ziqiang Zhu; Yiting Wang

doi:10.1117/1.OE.51.5.053202

7 May 2012 Pathological leucocyte segmentation algorithm based on hyperspectral imaging technique

Yana Guan, Qingli Li, Hongying Liu, Ziqiang Zhu, Yiting Wang

Author Affiliations +

Optical Engineering, Vol. 51, Issue 5, 053202 (May 2012). https://doi.org/10.1117/1.OE.51.5.053202

Abstract

White blood cells (WBC) are comparatively significant components in the human blood system, and they have a pathological relationship with some blood-related diseases. To analyze the disease information accurately, the most essential work is to segment WBCs. We propose a new method for pathological WBC segmentation based on a hyperspectral imaging system. This imaging system is used to capture WBC images, which is characterized by acquiring 1-D spectral information and 2-D spatial information for each pixel. A spectral information divergence algorithm is presented to segment pathological WBCs into four parts. In order to evaluate the performance of the new approach, K-means and spectral angle mapper-based segmental methods are tested in contrast on six groups of blood smears. Experimental results show that the presented method can segment pathological WBCs more accurately, regardless of their irregular shapes, sizes, and gray-values.

Citation Download Citation

Yana Guan, Qingli Li, Hongying Liu, Ziqiang Zhu, and Yiting Wang "Pathological leucocyte segmentation algorithm based on hyperspectral imaging technique," Optical Engineering 51(5), 053202 (7 May 2012). https://doi.org/10.1117/1.OE.51.5.053202

Published: 7 May 2012

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available