Functional localization microscopy by FLIM-FRET imaging (Conference Presentation)

Ani A. Jose

doi:10.1117/12.2650757

15 March 2023 Functional localization microscopy by FLIM-FRET imaging (Conference Presentation)

Ani A. Jose

Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC1238606 (2023) https://doi.org/10.1117/12.2650757
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Super-resolution imaging has become an essential tool used by the bioimaging community. In this study, we present a novel technique to resolve emitters located closer than the diffraction limit, by using the differences in their fluorescence lifetime. By calculating the centre of mass of the point spread function of the emitter on the timescale of the fluorescence decay, protein-protein interactions via FRET can be identified. This technique will also be able to distinguish the number of proximal neighbouring donors without the need to fit the time-resolved fluorescence decays.

Conference Presentation

Citation Download Citation

Ani A. Jose "Functional localization microscopy by FLIM-FRET imaging (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC1238606 (15 March 2023); https://doi.org/10.1117/12.2650757

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available