Spherical Aberration In Confocal Microscopy

T. Hellmuth; P. Seidel; A. Siegel

doi:10.1117/12.950300

9 February 1989 Spherical Aberration In Confocal Microscopy

T. Hellmuth, P. Seidel, A. Siegel

Proceedings Volume 1028, Scanning Imaging; (1989) https://doi.org/10.1117/12.950300
Event: 1988 International Congress on Optical Science and Engineering, 1988, Hamburg, Germany

Abstract

Scanning confocal microscopy has become an important tool in the field of optical sectioning and 3D-reconstruction of biological objects. Usually these samples are embedded in dispersive media, introducing depth dependent spherical aberration. Depth and lateral resolution are therefore impaired. We discuss this problem theoretically and show experimental results.

Citation Download Citation

T. Hellmuth, P. Seidel, and A. Siegel "Spherical Aberration In Confocal Microscopy", Proc. SPIE 1028, Scanning Imaging, (9 February 1989); https://doi.org/10.1117/12.950300

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