Hemoglobin spectra affect measurement of tissue oxygen saturation

Daniel Ostojic; Stefan Kleiser; Nassim Nasseri; Helene Isler; Felix Scholkmann; Tanja Karen; Martin Wolf

doi:10.1117/12.2292874

19 February 2018 Hemoglobin spectra affect measurement of tissue oxygen saturation

Daniel Ostojic, Stefan Kleiser, Nassim Nasseri, Helene Isler, Felix Scholkmann, Tanja Karen, Martin Wolf

Proceedings Volume 10486, Design and Quality for Biomedical Technologies XI; 104860T (2018) https://doi.org/10.1117/12.2292874
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Tissue oxygen saturation (StO₂) is a valuable clinical parameter e.g. for intensive care applications or monitoring during surgery. Studies showed that near-infrared spectroscopy (NIRS) based tissue oximeters of different brands give systematically different readings of StO₂. Usually these readings are linearly correlated and therefore StO₂ readings from one instrument can easily be converted to those of another instrument. However, it is interesting to understand why there is this difference. One reason may be that different brands employ different spectra of hemoglobin. The aim here was to investigate how these different absorption spectra of hemoglobin affect the StO₂ readings. Therefore, we performed changes in StO₂ in a phantom experiment with real human hemoglobin at three different concentrations (26.5, 45 and 70 μM): desaturation by yeast consuming the oxygen and re-saturation by bubbling oxygen gas. The partial pressure of O₂ in the liquid changed from at least 10 kPa to ~0 kPa and ISS OxiplexTS, a frequency-domain NIRS instrument, was used to monitor changes of StO₂. When we employed two different absorption spectra for hemoglobin, StO₂ values were comparable in the normal physiological range. However, particularly at high and low StO₂ values, a difference of >6% between these two spectra were noticed. Such a difference of >6% is substantial and relevant for medical applications. This may partly explain why different brands of NIRS instruments provide different StO₂ readings. The hemoglobin spectra are therefore a factor to be considered for future developments and applications of NIRS oximeters.

Citation Download Citation

Daniel Ostojic, Stefan Kleiser, Nassim Nasseri, Helene Isler, Felix Scholkmann, Tanja Karen, and Martin Wolf "Hemoglobin spectra affect measurement of tissue oxygen saturation", Proc. SPIE 10486, Design and Quality for Biomedical Technologies XI, 104860T (19 February 2018); https://doi.org/10.1117/12.2292874

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