Superresolution fluorescence imaging by pump-probe setup using repetitive stimulated transition process

Fumihiro Dake; Naoki Fukutake; Seri Hayashi; Yusuke Taki

doi:10.1117/12.2287325

20 February 2018 Superresolution fluorescence imaging by pump-probe setup using repetitive stimulated transition process

Fumihiro Dake, Naoki Fukutake, Seri Hayashi, Yusuke Taki

Author Affiliations +

Proceedings Volume 10500, Single Molecule Spectroscopy and Superresolution Imaging XI; 105000Q (2018) https://doi.org/10.1117/12.2287325
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We proposed superresolution nonlinear fluorescence microscopy with pump-probe setup that utilizes repetitive stimulated absorption and stimulated emission caused by two-color laser beams. The resulting nonlinear fluorescence that undergoes such a repetitive stimulated transition is detectable as a signal via the lock-in technique. As the nonlinear fluorescence signal is produced by the multi-ply combination of incident beams, the optical resolution can be improved. A theoretical model of the nonlinear optical process is provided using rate equations, which offers phenomenological interpretation of nonlinear fluorescence and estimation of the signal properties. The proposed method is demonstrated as having the scalability of optical resolution. Theoretical resolution and bead image are also estimated to validate the experimental result.

Conference Presentation

Citation Download Citation

Fumihiro Dake, Naoki Fukutake, Seri Hayashi, and Yusuke Taki "Superresolution fluorescence imaging by pump-probe setup using repetitive stimulated transition process", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000Q (20 February 2018); https://doi.org/10.1117/12.2287325

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