Fluorescence depolarization studies of heteroatom-doped CDs

Manoop Chenchiliyan; Hari Krishna Sadhanala; Kusha Sharma; Alix Le Marois; Aharon Gedanken; Dror Fixler

doi:10.1117/12.2508427

5 March 2019 Fluorescence depolarization studies of heteroatom-doped CDs

Manoop Chenchiliyan, Hari Krishna Sadhanala, Kusha Sharma, Alix Le Marois, Aharon Gedanken, Dror Fixler

Proceedings Volume 10891, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI; 108911J (2019) https://doi.org/10.1117/12.2508427
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In this work, we exploited time-resolved fluorescence polarization anisotropy (TRFA) to characterize un-doped and doped carbon dots (CDs). The rotational correlation time related to the size of the particle through classical Stokes-Einstein -Debye equation. The TRFA technique applied in this study achieves picoseconds time resolution, which approximately corresponds to the particle size determination at sub-nanometer precision. The calculated diameter of the CDs from the measured depolarization time constants in aqueous solution is well matches with the actual size of the CDs within the precision. This study proves that the TRFA method is highly complementary with other size determination techniques.

Citation Download Citation

Manoop Chenchiliyan, Hari Krishna Sadhanala, Kusha Sharma, Alix Le Marois, Aharon Gedanken, and Dror Fixler "Fluorescence depolarization studies of heteroatom-doped CDs", Proc. SPIE 10891, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI, 108911J (5 March 2019); https://doi.org/10.1117/12.2508427

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