Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues

Chih-Ju Lin; Jeon Woong Kang; Peter T. C. So; Chen-Yuan Dong

doi:10.1117/12.2545548

17 February 2020 Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues

Chih-Ju Lin, Jeon Woong Kang, Peter T. C. So, Chen-Yuan Dong

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441V (2020) https://doi.org/10.1117/12.2545548
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The formation and accumulation of advanced glycation end products (AGEs) contribute to diabetic complications such as retinopathy, neuropathy, nephropathy, and cardiovascular diseases. It is clear that the development of effective technique in AGEs detection and the establishing the correlation of measured AGE parameters to diabetic pathogenesis are invaluable in the monitoring of disease progression and drug discovery of anti-AGE compounds. Since some AGE are fluorescent, we propose to investigate the degree of tissue glycation in forming fluorescent AGEs (fAGEs). In this preliminary study, we will investigate the effects of glucose, fructose, and galactose, three of the most abundant dietary simple sugars, in fAGEs production. Excised tissues will be treated in solutions containing the three sugar types; multiphoton autofluorescence imaging will then be performed on the treated tissues to determine their autofluroescence levels.

Conference Presentation

Citation Download Citation

Chih-Ju Lin, Jeon Woong Kang, Peter T. C. So, and Chen-Yuan Dong "Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441V (17 February 2020); https://doi.org/10.1117/12.2545548

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