High-throughput multimodal Raman-fluorescence flow cytometry (Conference Presentation)

Matthew Lindley; Kotaro Hiramatsu; Fukashi Shibata; Tsuyoshi Takeshita; Shigeyuki Kawano; Keisuke Goda

doi:10.1117/12.2545022

10 March 2020 High-throughput multimodal Raman-fluorescence flow cytometry (Conference Presentation)

Matthew Lindley, Kotaro Hiramatsu, Fukashi Shibata, Tsuyoshi Takeshita, Shigeyuki Kawano, Keisuke Goda

Proceedings Volume 11250, High-Speed Biomedical Imaging and Spectroscopy V; 112500S (2020) https://doi.org/10.1117/12.2545022
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We present a high-throughput multimodal Raman-fluorescence flow cytometer that produces simultaneous Raman and fluorescence excitation spectra at a demonstrated throughput of 100 events per second. Our method combines Fourier-transform coherent anti-Stokes Raman scattering with Fourier-transform two-photon excitation fluorescence, which produces broadband Raman spectra that span the biological fingerprint region and single-detector multicolor fluorescence via spectral analysis. As a proof-of-principle demonstration, we perform multimodal label-free analysis of stress response in the microalga Haematococcus pluvialis by Raman measurement of its secondary metabolite astaxanthin and two-color fluorescence measurement of chlorophyll and fluorescent chlorophyll catabolites under different stress conditions.

Conference Presentation

Citation Download Citation

Matthew Lindley, Kotaro Hiramatsu, Fukashi Shibata, Tsuyoshi Takeshita, Shigeyuki Kawano, and Keisuke Goda "High-throughput multimodal Raman-fluorescence flow cytometry (Conference Presentation)", Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 112500S (10 March 2020); https://doi.org/10.1117/12.2545022

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KEYWORDS

Flow cytometry

Luminescence

Raman spectroscopy

Sensors

Biological research

CARS tomography

Femtosecond phenomena

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