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In this work, we study the effect of new iron oxide nanoparticles produced by the acoustoplasma method on the rate of formation of a fibrin gel in the enzymatic reaction of fibrinogen-thrombin. According to dynamic and static light scattering, we find that the addition of thrombin, preincubated with the nanoparticles (pH of the solution 7.5), to fibrinogen solution, accelerates the gelation reaction 30 times compared with the system without the addition of nanoparticles. Incubation of the nanoparticles with thrombin with decreased activity due to long storage leads to acceleration of the reaction in 4 times. The addition of thrombin, preincubated with the nanoparticles with less pH of the solution (5.5), to fibrinogen, in the opposite, slows down the reaction on the first stage of the process. During one hour after adding the thrombin-nanoparticles mixture to fibrinogen, the gelation reaction does not start. After this time, the reaction starts, but with the rate of 2 times less than without nanoparticles and 50 times less than with nanoparticles with pH 7.5. Such a difference in the biological effect of iron oxide nanoparticles on the rate of gelation under changing of their water environment pH is determined by the change in their surface charge (zeta-potential) and affinity for the thrombin enzyme.
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