Multi-wavelength digital holographic microscopy for bio-imaging and applications

Manoj Kumar; Osamu Matoba; Xiangyu Quan; Yasuhiro Awatsuji

doi:10.1117/12.2573229

15 June 2020 Multi-wavelength digital holographic microscopy for bio-imaging and applications

Manoj Kumar, Osamu Matoba, Xiangyu Quan, Yasuhiro Awatsuji

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210I (2020) https://doi.org/10.1117/12.2573229
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

A single-shot common-path off-axis multi-wavelength digital holographic microscopic (MW-DHM) system for phase imaging of biological specimens is proposed. The DHM system is based on a cube beam splitter which divides the object beam into two beams: one beam is spatially filtered at its Fourier plane to make a reference spherical beam and another beam acts as the object beam. Three wavelengths: 473, 532, and 632.8 nm are used to illuminate the object under observation. The obtained experimental results on various objects corroborate the imaging capability of the proposed single-shot common-path off-axis multi-wavelength MW-DHM system. The proposed system may open new possibilities in bio-imaging and real-time simultaneous measurement of various parameters including the thickness, refractive index, etc.

Citation Download Citation

Manoj Kumar, Osamu Matoba, Xiangyu Quan, and Yasuhiro Awatsuji "Multi-wavelength digital holographic microscopy for bio-imaging and applications", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210I (15 June 2020); https://doi.org/10.1117/12.2573229

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available