Fluorescence sectioning micro-endoscopy using hybrid illumination

Haw Hsiao; Chen-Yen Lin; Kuang-Yuh Huang; Yuan Luo

doi:10.1117/12.2573214

15 June 2020 Fluorescence sectioning micro-endoscopy using hybrid illumination

Haw Hsiao, Chen-Yen Lin, Kuang-Yuh Huang, Yuan Luo

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210M (2020) https://doi.org/10.1117/12.2573214
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

A widefield endoscope with optical sectioning capability using digital micro-mirror device (DMD) is proposed and demonstrated. With the addition of DMD, uniform and grid illumination pattern can be created for HiLo algorithm to remove out-of-focus noise in widefield images. By applying spectral filters to raw images, high frequency in-focus component of uniform illumination image and low frequency in-focus component of grid illumination image can be extracted, respectively. An in-focus image with full resolution can then be reconstructed by combining these two images. To verify the optical sectioning capability of the proposed system, 45μm fluorescent beads and mouse heart tissues were observed. The improvement of signal-to-noise ratio (SNR) can be obviously seen from the results.

Citation Download Citation

Haw Hsiao, Chen-Yen Lin, Kuang-Yuh Huang, and Yuan Luo "Fluorescence sectioning micro-endoscopy using hybrid illumination", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210M (15 June 2020); https://doi.org/10.1117/12.2573214

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