Three-dimensional tracking of moving Volvox by parallel phase-shifting digital holographic microscope

Junya Inamoto; Yuki Takase; Kohei Arao; Tomoyoshi Inoue; Kazuki Shimizu; Kenzo Nishio; Osamu Matoba; Toshihiro Kubota; Yasuhiro Awatsuji

doi:10.1117/12.2573282

15 June 2020 Three-dimensional tracking of moving Volvox by parallel phase-shifting digital holographic microscope

Junya Inamoto, Yuki Takase, Kohei Arao, Tomoyoshi Inoue, Kazuki Shimizu, Kenzo Nishio, Osamu Matoba, Toshihiro Kubota, Yasuhiro Awatsuji

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Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210V (2020) https://doi.org/10.1117/12.2573282
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

We report the three-dimensional (3D) trajectory of a Volvox moving in water was recorded by parallel phase-shifting digital holographic microscope providing 10X magnification. The recording frame rate, the shutter speed, and the total recording time were 1000 fps, 0.25 ms, and 2.1 s, respectively. In the reconstructed phase image of the Volvox, the shape of the Volvox is regarded as a circle. The lateral coordinates of the Volvox were determined as the center of the circle. The depth coordinates of the Volvox were determined as the propagation distance where the edge of the Volvox in the reconstructed amplitude image was clearest while the propagation distance was varied. We successfully demonstrated the 3D tracking of curvedly moving Volvox.

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Junya Inamoto, Yuki Takase, Kohei Arao, Tomoyoshi Inoue, Kazuki Shimizu, Kenzo Nishio, Osamu Matoba, Toshihiro Kubota, and Yasuhiro Awatsuji "Three-dimensional tracking of moving Volvox by parallel phase-shifting digital holographic microscope", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210V (15 June 2020); https://doi.org/10.1117/12.2573282

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KEYWORDS

Digital holography

3D image reconstruction

Microscopes

Holograms

Holography

Phase shifts

3D metrology

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