Recent progress of 3D fluorescence and phase imaging techniques

Osamu Matoba; Manoj Kumar; Xiangyu Quan; Sudheesh K. Rajput; Yasuhiro Awatsuji

doi:10.1117/12.2573920

10 October 2020 Recent progress of 3D fluorescence and phase imaging techniques

Osamu Matoba, Manoj Kumar, Xiangyu Quan, Sudheesh K. Rajput, Yasuhiro Awatsuji

Proceedings Volume 11551, Holography, Diffractive Optics, and Applications X; 115510U (2020) https://doi.org/10.1117/12.2573920
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

In this presentation, we briefly review the fluorescence and the phase imaging techniques based on computational imaging such as digital holography and transport of intensity equation. These techniques uses an image sensor to capture the hologram or the intensity distribution and it enables more faster recording than the point-scanning methods. The presented techniques have also an advantage to refocus the images by applying an appropriate distance. Some experimental results are provided to show the effectiveness of the methods. Multimodal three-dimensional imaging is also presented.

Conference Presentation

Citation Download Citation

Osamu Matoba, Manoj Kumar, Xiangyu Quan, Sudheesh K. Rajput, and Yasuhiro Awatsuji "Recent progress of 3D fluorescence and phase imaging techniques", Proc. SPIE 11551, Holography, Diffractive Optics, and Applications X, 115510U (10 October 2020); https://doi.org/10.1117/12.2573920

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