A commercialized digital holographic microscope with complete software supporting

Zhuoshi Li; Yao Fan; Jiasong Sun; Chao Zuo; Qian Chen

doi:10.1117/12.2581220

7 October 2020 A commercialized digital holographic microscope with complete software supporting

Zhuoshi Li, Yao Fan, Jiasong Sun, Chao Zuo, Qian Chen

Proceedings Volume 11571, Optics Frontier Online 2020: Optics Imaging and Display; 115711C (2020) https://doi.org/10.1117/12.2581220
Event: Optics Frontiers Online 2020: Optics Imaging and Display (OFO-1), 2020, Shanghai, China

Abstract

A commercialized digital holographic microscope with complete software supporting is composed of software and hardware, and also has quantitative phase recovery and phase compensation algorithm support. The calculation speed can reach thirty frames per second, and the resolution can reach 0.775 μm under a 20 times 0.4 NA objective lens, which can realize subcellular three-dimensional reconstruction and thickness distribution measurement of transparent samples in real-time and high resolution. This paper describes the optical path geometry, algorithm flow and image calibration of the system, including the phase aberration compensation algorithm based on principal component analysis (PCA), unwrapping algorithm based on reliability analysis and subpixel displacement technology. These algorithms are processed by software in real time and can be adjusted flexibly. In addition, the whole system is tested by using phase resolution board. Quantitative phase recovery, three-dimensional structure display and differential interference contrast (DIC) display were performed for human cervical cancer cells and human oral epithelial cells, and quantitative profile measurement was conducted for pollen.

Citation Download Citation

Zhuoshi Li, Yao Fan, Jiasong Sun, Chao Zuo, and Qian Chen "A commercialized digital holographic microscope with complete software supporting", Proc. SPIE 11571, Optics Frontier Online 2020: Optics Imaging and Display, 115711C (7 October 2020); https://doi.org/10.1117/12.2581220

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available