Multimodal high-resolution system for embryonic imaging combining optical coherence tomography and light sheet fluorescence microscopy

Behzad Khajavi; Ruijiao Sun; Harshdeep S. Chawla; Henry H. Le; Manmohan Singh; Alexander W. Schill; David Mayerich; Mary E. Dickinson; Kirill V. Larin

doi:10.1117/12.2588572

5 March 2021 Multimodal high-resolution system for embryonic imaging combining optical coherence tomography and light sheet fluorescence microscopy

Behzad Khajavi, Ruijiao Sun, Harshdeep S. Chawla, Henry H. Le, Manmohan Singh, Alexander W. Schill, David Mayerich, Mary E. Dickinson, Kirill V. Larin

Author Affiliations +

Proceedings Volume 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII; 116410V (2021) https://doi.org/10.1117/12.2588572
Event: SPIE BiOS, 2021, Online Only

Abstract

We developed a high-resolution multimodal system for mouse embryonic imaging that combines Optical Coherence Tomography (OCT) and Light Sheet Fluorescence Microscopy (LSFM). LSFM Illumination is restricted to fluorophores in the focal volume, and collecting the light using a microscope objective increases the signal from that plane and reduces the noise coming from outside of the plane. Colinearly aligning this modality with the OCT beam allows one to acquire the structural information from the same plane that is illuminated by the LSFM beam. A 3D image of 9.5 day mouse embryo was captured using this multimodal system.

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Citation Download Citation

Behzad Khajavi, Ruijiao Sun, Harshdeep S. Chawla, Henry H. Le, Manmohan Singh, Alexander W. Schill, David Mayerich, Mary E. Dickinson, and Kirill V. Larin "Multimodal high-resolution system for embryonic imaging combining optical coherence tomography and light sheet fluorescence microscopy", Proc. SPIE 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII, 116410V (5 March 2021); https://doi.org/10.1117/12.2588572

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