Real time and label-free detection of C-reactive protein in serum by long period grating in double cladding fiber

Flavio Esposito; Lucia Sansone; Anubhav Srivastava; Francesco Baldini; Stefania Campopiano; Francesco Chiavaioli; Michele Giordano; Ambra Giannetti; Agostino Iadicicco

doi:10.1117/12.2589969

18 April 2021 Real time and label-free detection of C-reactive protein in serum by long period grating in double cladding fiber

Flavio Esposito, Lucia Sansone, Anubhav Srivastava, Francesco Baldini, Stefania Campopiano, Francesco Chiavaioli, Michele Giordano, Ambra Giannetti, Agostino Iadicicco

Author Affiliations +

Proceedings Volume 11772, Optical Sensors 2021; 117720Q (2021) https://doi.org/10.1117/12.2589969
Event: SPIE Optics + Optoelectronics, 2021, Online Only

Abstract

Here, we present a novel label-free biosensor based on fiber optic technology which was tested for the detection of a serum inflammatory marker, the C-reactive protein (CRP). The biosensor is based on a long period grating (LPG) inscribed in a double cladding fiber (DCF) having a W-type refractive index profile. Such DCF fiber permits to tune the sensor working point to the so-called mode transition region through etching of the fiber outer cladding. Therefore, a significant enhancement of the refractive index sensitivity, as well as visibility of the grating spectral features were attained since the mode transition was induced in all-silica fiber structure. Subsequently, the so-prepared LPG was coated with a nano-scale layer of graphene oxide, providing carboxylic functional groups for the covalent immobilization of the biological recognition element for the CRP. As a result, a remarkable limit of detection of 320 pg/mL and a large working range of clinical relevance (0.002-100 μg/mL) were achieved during the real time detection of CRP in human serum.

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Flavio Esposito, Lucia Sansone, Anubhav Srivastava, Francesco Baldini, Stefania Campopiano, Francesco Chiavaioli, Michele Giordano, Ambra Giannetti, and Agostino Iadicicco "Real time and label-free detection of C-reactive protein in serum by long period grating in double cladding fiber", Proc. SPIE 11772, Optical Sensors 2021, 117720Q (18 April 2021); https://doi.org/10.1117/12.2589969

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