Validation of dynamic contrast-enhanced bone blood flow imaging technique with fluorescent microspheres

Xinyue Han; Valentin Demidov; Dennis Wirth; Brook Byrd; Scott Davis; Leah Gitajn; Jonathan Elliott

doi:10.1117/12.2608228

4 March 2022 Validation of dynamic contrast-enhanced bone blood flow imaging technique with fluorescent microspheres

Xinyue Han, Valentin Demidov, Dennis Wirth, Brook Byrd, Scott Davis, Leah Gitajn, Jonathan Elliott

Proceedings Volume 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII; 119430H (2022) https://doi.org/10.1117/12.2608228
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

In orthopedic trauma surgery, timely assessment of bone tissue perfusion plays a vital role in the successful treatment outcome. Fluorescence-guidance is gaining increased surgical interest, especially with respect to hemodynamic assessment of bone. Intraoperative dynamic contrast-enhanced fluorescence imaging (DCE-FI) not only enables visualization of the perfused areas of the injured bone, but with subsequent analysis using kinetic models, may also provide a valuable quantitative bone blood flow information to a surgeon. In this study, we are validating this quantitative approach with a modified fluorescent microsphere (FM) technique using a custom-built four-channel imaging cryomacrotome. We demonstrate that FMs of four different colors can be accurately detected in controlled phantoms and evaluate their detection accuracy in real blood samples. In a rabbit model of orthopaedic trauma, we show that blood flow measurements using the DCE-FI technique can be compared with the FM technique. This feasibility pilot study provides the groundwork for investigation of the correlation between bone perfusion measurements using DCE-FI and using fluorescent microspheres, in units of ml/min/100g.

Conference Presentation

Citation Download Citation

Xinyue Han, Valentin Demidov, Dennis Wirth, Brook Byrd, Scott Davis, Leah Gitajn, and Jonathan Elliott "Validation of dynamic contrast-enhanced bone blood flow imaging technique with fluorescent microspheres", Proc. SPIE 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, 119430H (4 March 2022); https://doi.org/10.1117/12.2608228

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