Label-free multiscale dynamic imaging using 3D phase contrast and deep UV microscopy

Francisco E. Robles

doi:10.1117/12.2617118

4 March 2022 Label-free multiscale dynamic imaging using 3D phase contrast and deep UV microscopy

Francisco E. Robles

Proceedings Volume 11944, Multiscale Imaging and Spectroscopy III; 1194404 (2022) https://doi.org/10.1117/12.2617118
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

In this work we will discuss novel tools for label-free subcellular dynamic imaging. We will explore dynamics using two orthogonal modalities: First, we will look at dynamic information derived from refractive index fluctuations using an emerging 3D quantitative phase imaging methods called quantitative oblique back illumination microscopy (qOBM). Next, we will look at dynamic information derived from the excitation coefficient of endogenous biomolecules in cells using multi-spectral deep-UV microscopy. Dynamics at multiple time-scales will be explored and their unique attributes and applications in biomedicine will be discussed.

Conference Presentation

Citation Download Citation

Francisco E. Robles "Label-free multiscale dynamic imaging using 3D phase contrast and deep UV microscopy", Proc. SPIE 11944, Multiscale Imaging and Spectroscopy III, 1194404 (4 March 2022); https://doi.org/10.1117/12.2617118

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