Multimodal microscopy from tomographic diffraction microscopy acquisitions

Riadh Abbessi; Steve Laroche; Nicolas Verrier; Jean-Baptiste Courbot; Matthieu Debailleul; Olivier Haeberlé

doi:10.1117/12.2618295

20 May 2022 Multimodal microscopy from tomographic diffraction microscopy acquisitions

Riadh Abbessi, Steve Laroche, Nicolas Verrier, Jean-Baptiste Courbot, Matthieu Debailleul, Olivier Haeberlé

Proceedings Volume 12136, Unconventional Optical Imaging III; 121360S (2022) https://doi.org/10.1117/12.2618295
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Conventional optical microscopy is one of the most commonly used method in biological imaging studies. However, intensity-based imaging techniques exhibit poor imaging contrasts when biological samples are considered. Several methods, like phase contrast microscopy or darkfield microscopy, have been proposed to overcome these limitations. A better qualitative information can then be extracted at the cost of experimental devices multiplicity. On the other hand, quantitative phase imaging techniques like Tomographic Diffraction Microscopy (TDM) make it possible to extract full 3D information about the light field emerging from the investigated sample. In this article, we propose to take benefits of TDM capabilities to simulate the behavior of conventional optical microscope, making TDM and universal microscopy imaging platform.

Citation Download Citation

Riadh Abbessi, Steve Laroche, Nicolas Verrier, Jean-Baptiste Courbot, Matthieu Debailleul, and Olivier Haeberlé "Multimodal microscopy from tomographic diffraction microscopy acquisitions", Proc. SPIE 12136, Unconventional Optical Imaging III, 121360S (20 May 2022); https://doi.org/10.1117/12.2618295

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