Polarization imaging of biological samples with spatial filtering

Zhidi Liu; Nan Zeng; Jiawei Song; Hui Ma

doi:10.1117/12.2643386

19 December 2022 Polarization imaging of biological samples with spatial filtering

Zhidi Liu, Nan Zeng, Jiawei Song, Hui Ma

Proceedings Volume 12320, Optics in Health Care and Biomedical Optics XII; 1232009 (2022) https://doi.org/10.1117/12.2643386
Event: SPIE/COS Photonics Asia, 2022, Online Only

Abstract

Polarization measurement technology can detect the microscopic structure information from biological samples. However, for biological samples with weak scattering effect, the optical signal obtained by the detector will contain a large amount of zero-frequency light without scattering, which makes it difficult to effectively extract the polarization information of the samples with weak scattering effect. Therefore, in this study, we introduce spatial filtering technique into polarization measurement. In the experimental setup, a spatial filter is placed in the rear focal plane of the objective lens to block the zero-frequency light, so as to realize the selection of scattered photons. A red LED with the wavelength of 633nm was used as the lighting source. The measurement of polarization information is based on the dual-rotating quarter-wave plate method. Considering the widespread presence of spherical and columnar scatterers in biological systems, Hacat cells and biological fibers were selected for the experiment. By comparing with the polarization data without spatial filtering, we found that the polarization parameter obtained after the high pass filtering measurement can better characterize the samples.

Conference Presentation

Citation Download Citation

Zhidi Liu, Nan Zeng, Jiawei Song, and Hui Ma "Polarization imaging of biological samples with spatial filtering", Proc. SPIE 12320, Optics in Health Care and Biomedical Optics XII, 1232009 (19 December 2022); https://doi.org/10.1117/12.2643386

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available