Rapid, variable aperture approach to quantify depth of fluorescence in a heterogenous medium

Cody C. Rounds; Jaron de Wit; Jasper Vonk; Floris Voskuil; Jennifer Vorjohan; Sophia Nelson; Brooke Villinski; Allyson Trang; Max J. H. Witjes; Kenneth M. Tichauer

doi:10.1117/12.2650919

14 March 2023 Rapid, variable aperture approach to quantify depth of fluorescence in a heterogenous medium

Cody C. Rounds, Jaron de Wit, Jasper Vonk, Floris Voskuil, Jennifer Vorjohan, Sophia Nelson, Brooke Villinski, Allyson Trang, Max J. H. Witjes, Kenneth M. Tichauer

Author Affiliations +

Proceedings Volume 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX; 1236105 (2023) https://doi.org/10.1117/12.2650919
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Post-operative assessment of resected tumor margins is critical to ensure the entirety of malignant tissue has been removed from a patient. Microscopic assessment of tissue post-excision is the current gold standard, however the long wait times for proper specimen evaluation limit a surgeon’s ability to be certain they obtained clear margins. To address this need, fluorescence-guided surgery approaches are under development that can yield molecular contrast between healthy and malignant tissues intraoperatively. In head and neck cancer specifically, heterogenous optical properties lead to poor identification in margins greater than 1 mm thick when viewed with single projections. Thus, we demonstrate the use of variable aperture approach to decrease the effects of local optical property variations in the imaged specimen. Here we use Monte Carlo simulations to verify the utility of the idea in a homogenous medium as well in a medium with heterogenous properties. We demonstrate that a ratio metric approach can provide near identical depth discrimination as a single projection in a homogenous medium and is further capable of reducing pixel variability due to local optical properties in a heterogenous medium than a single projection alone.

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Cody C. Rounds, Jaron de Wit, Jasper Vonk, Floris Voskuil, Jennifer Vorjohan, Sophia Nelson, Brooke Villinski, Allyson Trang, Max J. H. Witjes, and Kenneth M. Tichauer "Rapid, variable aperture approach to quantify depth of fluorescence in a heterogenous medium", Proc. SPIE 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX, 1236105 (14 March 2023); https://doi.org/10.1117/12.2650919

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