Quantification of inflammation and toxicity-induced effects on living cells and in histological ex vivo tissues utilizing digital holographic phase microscopy

Björn Kemper

doi:10.1117/12.2673871

11 August 2023 Quantification of inflammation and toxicity-induced effects on living cells and in histological ex vivo tissues utilizing digital holographic phase microscopy

Björn Kemper

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 1262207 (2023) https://doi.org/10.1117/12.2673871
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

In an overview, Digital Holographic Microscopy (DHM) for usage in a biomedical laboratory environment and the application of DHM-based Quantitative Phase Imaging (QPI) for quantification of inflammation and toxicity related effects in tissue sections, cell cultures and blood cells are presented. First, the capabilities to determine inflammation as well as nanomaterial induced pathological alterations in dissected ex vivo colon and lung tissues are illustrated. Then, results from cell culture-based time-lapse growth monitoring assays and perioperative observation of living primary human leucocytes demonstrate DHM as a cytometric tool for in vitro toxicity testing and temporal disease course characterization.

Conference Presentation

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Björn Kemper "Quantification of inflammation and toxicity-induced effects on living cells and in histological ex vivo tissues utilizing digital holographic phase microscopy", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 1262207 (11 August 2023); https://doi.org/10.1117/12.2673871

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