Simultaneous fluorescence microscopy and spectroscopy of oral squamous cell carcinoma, oral dysplasia, and normal tissue

Pramila Thapa; Sunil Bhatt; Priyanka Mann; Vivek Nayyar; Deepika Mishra; Dalip Singh Mehta

doi:10.1117/12.2670948

11 August 2023 Simultaneous fluorescence microscopy and spectroscopy of oral squamous cell carcinoma, oral dysplasia, and normal tissue

Pramila Thapa, Sunil Bhatt, Priyanka Mann, Vivek Nayyar, Deepika Mishra, Dalip Singh Mehta

Author Affiliations +

Proceedings Volume 12627, Translational Biophotonics: Diagnostics and Therapeutics III; 126271Z (2023) https://doi.org/10.1117/12.2670948
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Complete removal of the oral cancer is very crucial and depends upon the early-stage detection and regular screening. Oral squamous cell carcinoma (OSCC) is the main cancer among oral cancer and shows a high fatality rate. Early-stage screening could be possible with the help of optical techniques and can lead to low fatality. Present manuscript deals with two modalities fluorescence microscopy and fluorescence spectroscopy of OSCC, precancerous and normal tissues. A GRIN lens based micro-endoscope is used for the fluorescence microscopic imaging and spectroscopy of the oral cancer tissue and precancerous tissues. Fluorescence technique provides the chemical compositions of normal, precancerous and cancerous tissue as the microenvironment is changed in each case. Fluorescence microscopic imaging is done using micro-endo-spectroscopic system which gives quantitative fluorescence contrast with simultaneous fluorescence spectroscopy. Integration of both modalities in single study shows potentiality for OSCC screening. A significant change in fluorescence contrast of OSCC, precancerous and normal tissue is observed with their adjacent fluorescence spectra. In fluorescence spectra a red-shift is recorded for OSCC, precancerous and normal tissue. Fluorescence imaging gives the statistical quantitative parameters based on the fluorescence contrast and fluorescence spectroscopy gives the information about molecular transitions present in cancerous and normal tissue.

Citation Download Citation

Pramila Thapa, Sunil Bhatt, Priyanka Mann, Vivek Nayyar, Deepika Mishra, and Dalip Singh Mehta "Simultaneous fluorescence microscopy and spectroscopy of oral squamous cell carcinoma, oral dysplasia, and normal tissue", Proc. SPIE 12627, Translational Biophotonics: Diagnostics and Therapeutics III, 126271Z (11 August 2023); https://doi.org/10.1117/12.2670948

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