Multispectral digital holographic microscopy with tunable supercontinuum lasers

Tobias Kurtz; Álvaro Barroso; José Ángel Picazo-Bueno; Steffi Ketelhut; Jürgen Schnekenburger; Björn Kemper

doi:10.1117/12.3003662

12 March 2024 Multispectral digital holographic microscopy with tunable supercontinuum lasers

Tobias Kurtz, Álvaro Barroso, José Ángel Picazo-Bueno, Steffi Ketelhut, Jürgen Schnekenburger, Björn Kemper

Proceedings Volume 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; 1284807 (2024) https://doi.org/10.1117/12.3003662
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Supercontinuum (SC) lasers in combination with tunable acousto-optic filters facilitate multiwavelength digital holographic microscopy (DHM) with a broad spectral range. However, to achieve optimized quantitative phase imaging (QPI) in off-axis DHM the coherence properties of the tunable light source and the holographic carrier interference fringe pattern formation have to be considered as they are wavelength dependent. We thus determined experimentally the wavelength dependency of coherence length and off-axis carrier fringe frequency in a Michelson interferometer-based multi-spectral DHM setup with a continuously tunable SC laser and moreover analyzed the impact of both parameters on hologram formation and QPI image quality. The results are found in good agreement with theoretical predictions and provide essential information for a reliable adjustment of SC laser based multispectral off-axis DHM systems. Finally, the application of multispectral-DHM for reduction of coherence induced disturbances in quantitative phase images of living pancreatic tumor cells in illustrated.

Conference Presentation

Citation Download Citation

Tobias Kurtz, Álvaro Barroso, José Ángel Picazo-Bueno, Steffi Ketelhut, Jürgen Schnekenburger, and Björn Kemper "Multispectral digital holographic microscopy with tunable supercontinuum lasers", Proc. SPIE 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, 1284807 (12 March 2024); https://doi.org/10.1117/12.3003662

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