High spatial and temporal resolution in the optical investigation of biological objects

Tobias Damm; Michael Kempe; Uwe Stamm; K. P. Stolberg; Heidrun Wabnitz

doi:10.1117/12.57346

1 May 1991 High spatial and temporal resolution in the optical investigation of biological objects

Tobias Damm, Michael Kempe, Uwe Stamm, K. P. Stolberg, Heidrun Wabnitz

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57346
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

If laser scanning microscopy with enhanced spatial or temporal resolution is performed on sensible biological samples it is essential to prevent damage or substantial alteration of the object due to intense laser illumination. Considering a simplified model for temperature rise and photochemical reactions conclusions concerning scanning and measuring conditions are drawn. A method for improving the spatial resolution of the laser scanning microscope by image processing is described. This method is based on a local deconvolution procedure and yields an improvement of the resolution of about 1. 7 in a discussed example. It is applied to images of chromosomes. For time-resolved fluorescence microscopy on the basis of time-correlated single photon counting methods for obtaining images of fluorescence decay parameters under the condition of weak fluorescence intensity are discussed. As an example intensity and relaxation time images of a tumour cell incubated with HpD are presented. 1.

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Tobias Damm, Michael Kempe, Uwe Stamm, K. P. Stolberg, and Heidrun Wabnitz "High spatial and temporal resolution in the optical investigation of biological objects", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57346

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