Circular polarization effects in the light scattering from single and suspensions of dinoflagellates

Daniel B. Shapiro; Arlon J. Hunt; Mary S. Quinby-Hunt; Patricia G. Hull

doi:10.1117/12.49258

1 December 1991 Circular polarization effects in the light scattering from single and suspensions of dinoflagellates

Daniel B. Shapiro, Arlon J. Hunt, Mary S. Quinby-Hunt, Patricia G. Hull

Proceedings Volume 1537, Underwater Imaging, Photography, and Visibility; (1991) https://doi.org/10.1117/12.49258
Event: San Diego, '91, 1991, San Diego, CA, United States

Abstract

Light scattering measurements were performed on single, immobilized dinoflagellates as well as on suspensions of the species Prorocentrum micans. The normalized Mueller scattering matrix element S₁₄, which indicates an ability to depolarize circularly polarized light, is reported for both cases. The measurements involving single cells were performed on Prorocentrum micans, Gonyaulax polyhedra, and Crypthecodinium cohnii. The results show that the previously reported large S₁₄ signal is not peculiar to P. micans. Time-dependent measurements of live cultures of P. micans show a large, high-frequency S₁₄ signal. This signal is a diurnal function of the time of day, with a maximum at midnight. Investigations of the relationship between single cell and suspension measurements reveal that the large angle- dependent S₁₄ peaks from immobilized single dinoflagellates are responsible for a large time-dependent S₁₄ signal at 90 degree(s) in suspension measurements. The results of these experiments provide further evidence for the hypothesis that the chromosomes of the dinoflagellates are responsible for the large observed S₁₄ signals. The unusual depolarization properties of dinoflagellates should be considered when using polarized light to enhance image contrast in underwater imaging.

Citation Download Citation

Daniel B. Shapiro, Arlon J. Hunt, Mary S. Quinby-Hunt, and Patricia G. Hull "Circular polarization effects in the light scattering from single and suspensions of dinoflagellates", Proc. SPIE 1537, Underwater Imaging, Photography, and Visibility, (1 December 1991); https://doi.org/10.1117/12.49258

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