Picosecond polarization microfluorometry of hematoporphyrin in single living cell

Oleg I. Lobanov; Serge Yu. Arjantsev; D. E. Akindinov

doi:10.1117/12.146123

17 June 1993 Picosecond polarization microfluorometry of hematoporphyrin in single living cell

Oleg I. Lobanov, Serge Yu. Arjantsev, D. E. Akindinov

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146123
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The method of time-resolved polarization fluorescence spectroscopy combined with picosecond laser microscopy allows the development of adequate equipment for quantitative detection of porphyrin monomers, dimers and aggregates. In addition to steady state fluorescence microscopy which has been applied to study the intracellular distribution of photosensitizing porphyrins, time-resolved polarization microfluorometry provides more exact information about the size and shape of the porphyrins aggregates in different sites of the single living cell.

Citation Download Citation

Oleg I. Lobanov, Serge Yu. Arjantsev, and D. E. Akindinov "Picosecond polarization microfluorometry of hematoporphyrin in single living cell", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146123

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