Fluorescence spectroscopy and imaging of pH in vivo using a dual emission fluorophore (C-SNAFL-1)

Serge R. Mordon; Jean-Marie Devoisselle; Sylvie Soulie-Begu

doi:10.1117/12.198713

4 January 1995 Fluorescence spectroscopy and imaging of pH in vivo using a dual emission fluorophore (C-SNAFL-1)

Serge R. Mordon, Jean-Marie Devoisselle, Sylvie Soulie-Begu

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198713
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Fluorescent intracellular pH indicators have been widely applied in recent years to monitor changes in intracellular pH. More recently, we have proposed the use of dual-excitation pH-fluorescent indicators (5,6-CF: 5,6- carboxyfluorescein or BCECF: 2',7'-bis-carboxyethyl-5,6-carboxy-fluorescein) to monitor pH on animals. This study aimed to evaluate a dual-emission fluorophore (C-SNAFL-1: 5' (and 6') carboxy-seminaphthofluorescein) for pH monitoring in-vivo. In that case, one excitation wavelength and two emissions are chosen to give a pH-dependent emission intensity ratio.

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Serge R. Mordon, Jean-Marie Devoisselle, and Sylvie Soulie-Begu "Fluorescence spectroscopy and imaging of pH in vivo using a dual emission fluorophore (C-SNAFL-1)", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198713

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KEYWORDS

Luminescence

Tissues

Tumors

In vivo imaging

Fluorescence spectroscopy

Glucose

Spectroscopy

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