Immobilized fluorescent dyes for sensitive pH measurements on enamel surfaces with fiber optics

A. Rumphorst; Stefan Seeger; H. Duschner

doi:10.1117/12.229508

8 January 1996 Immobilized fluorescent dyes for sensitive pH measurements on enamel surfaces with fiber optics

A. Rumphorst, Stefan Seeger, H. Duschner

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229508
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Information on the pH directly on surfaces of dental enamel is an important aspect in research on tooth decay. As an alternative to pH-electrodes our approach to the problem is the optical determination of pH by pH sensitive fluorescent dyes immobilized to tooth surfaces. In this study a model for measuring pH either on aminated cellulose substrates or on enamel (in vitro) with a fluorescein type dye is presented. The experimental realization is a fiber optic sensor with a nitrogen-pumped dye laser system and photodiode for the detection of the emitted fluorescence light. The surface pH values in the range between 4 and 7 were derived from the ratios of the excitation bands at 490 nm and 460 nm.

Citation Download Citation

A. Rumphorst, Stefan Seeger, and H. Duschner "Immobilized fluorescent dyes for sensitive pH measurements on enamel surfaces with fiber optics", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229508

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available