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Ion-selective bulk optode membranes have been combined with enzymatic reactions for the determination of neutral analytes in clinical applications. A new development in membrane technology has been introduced, using reverse micelles to entrap the biocatalyst in bulk polymeric membranes. The use of such reverse micellar systems allows the design of a single layer biosensor where the recognition process as well as the chemical transduction into an optical signal take place in the same sensing layer. Urea-sensitive micellar bio-optode membranes have been realized; dynamic range, response behavior, long-term stability as well as the operational lifetime are discussed.
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Eva Vaillo, Ursula E. Spichiger-Keller, "Micellar bio-optode membranes," Proc. SPIE 2631, Medical and Fiber Optic Sensors and Delivery Systems, (3 January 1995); https://doi.org/10.1117/12.229175