Fundamental system for biosensor characterization: application to surface plasmon resonance (SPR)

Clement E. Furlong; Richard G. Woodbury; Sinclair S. Yee; Timothy M. Chinowsky; Richard Carr; Jerome L. Elkind; Kari A. Kukanskis; Dwight U. Bartholomew; Jose L. Melendez

doi:10.1117/12.260594

10 December 1996 Fundamental system for biosensor characterization: application to surface plasmon resonance (SPR)

Clement E. Furlong, Richard G. Woodbury, Sinclair S. Yee, Timothy M. Chinowsky, Richard Carr, Jerome L. Elkind, Kari A. Kukanskis, Dwight U. Bartholomew, Jose L. Melendez

Author Affiliations +

Proceedings Volume 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII; (1996) https://doi.org/10.1117/12.260594
Event: SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, 1996, Denver, CO, United States

Abstract

The aim of the described research is to develop a general system for characterizing and developing signal transduction systems for microbiosensors. The approach that we are using is applicable to signal transduction systems based on surface plasmon resonance, chemiluminescence, fluorescence, mass as well as other phenomena. The specific goal of our approach is to develop a general system that will allow for the systematic characterization of the effects of the affinity of the sensor specificity element for the target analyte, the effect of analyte mass on signal size and the general performance of the sensor system with respect to sensitivity and selectivity. At the same, time this system should allow for the characterization of the distribution of biospecificity elements on the sensor surface. We chose the anti-fluorescein monoclonal antibody approach for this development system, since the antigen fluorescein can be attached to many different molecules and organisms through free amine groups via reaction with fluorescein isothiocyanate. Also, well characterized monoclonal antibodies with a broad range of Kd values are available. We also describe rapid procedures for generating proteins for use in biosensor applications.

Citation Download Citation

Clement E. Furlong, Richard G. Woodbury, Sinclair S. Yee, Timothy M. Chinowsky, Richard Carr, Jerome L. Elkind, Kari A. Kukanskis, Dwight U. Bartholomew, and Jose L. Melendez "Fundamental system for biosensor characterization: application to surface plasmon resonance (SPR)", Proc. SPIE 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII, (10 December 1996); https://doi.org/10.1117/12.260594

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