Optical tweezers as a tool for the functional analysis of neuronal cell membrane receptors

Martin Zahn; Bernhard Goetz; Andreas Faissner; Stefan Seeger

doi:10.1117/12.301125

1 January 1998 Optical tweezers as a tool for the functional analysis of neuronal cell membrane receptors

Martin Zahn, Bernhard Goetz, Andreas Faissner, Stefan Seeger

Proceedings Volume 3199, Biomedical Systems and Technologies II; (1998) https://doi.org/10.1117/12.301125
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Recognition molecules play important regulatory roles during pattern formation of the nervous system. We here show that optical tweezers can successfully be used to functionally and quantitatively compare neuron interactions with glycoproteins of the extracellular matrix. We measured the forces of interactions between tenascin-C, laminin-1 and fibronectin coated polystyrol beads and the cell membranes of different nervous cell-types. This was achieved by applying the optical tweezers as picotensometer to pull the adhering beads with varying axial forces. To this aim, the minimal laser powers for capturing polystyrene beads in different solutions were measured. With the corresponding Archimedes Forces the acting forces in the range of pN were calculated. The glycoproteins showed significantly different behavior. Specific binding was shown by antibody experiments, in which the binding forces were significantly reduced for the corresponding antigens but not for the other glycoproteins. Together with experiments concerning the moving within the plane of the membrane and the cleavage with glycosyl-phosphatidyl-inositol-anchors specific phospholipase-C we suggest that these glycoproteins interact with neurons by different mechanisms.

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Martin Zahn, Bernhard Goetz, Andreas Faissner, and Stefan Seeger "Optical tweezers as a tool for the functional analysis of neuronal cell membrane receptors", Proc. SPIE 3199, Biomedical Systems and Technologies II, (1 January 1998); https://doi.org/10.1117/12.301125

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KEYWORDS

Neurons

Optical tweezers

Receptors

Molecules

Proteins

Brain

Calibration

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