IR spectroscopy and IR microscopy of human breast tumors, xenografted breast tumors, and breast tumor cell lines

Heinz Fabian; Ralf Wessel; Michael Jackson; Arnfried Schwartz; Peter Lasch; Iduna Fichtner; Henry H. Mantsch; Dieter Naumann

doi:10.1117/12.306082

24 April 1998 IR spectroscopy and IR microscopy of human breast tumors, xenografted breast tumors, and breast tumor cell lines

Heinz Fabian, Ralf Wessel, Michael Jackson, Arnfried Schwartz, Peter Lasch, Iduna Fichtner, Henry H. Mantsch, Dieter Naumann

Author Affiliations +

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306082
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

IR spectra of breast tumor cell lines and breast tumor tissues have been measured. IR measurements of tumor cells revealed that approximately 15 cells are necessary to obtain spectra of good signal-to-noise ratio using an IR microspectrometer equipped with a conventional IR thermal source. Comparative studies of human breast tumor cell line suspensions demonstrated that MCF-7 cells and drug-resistant NCI/ADR cells can be differentiated based on their IR spectra. The most striking differences between MCF-7 and NCI/ADR were found in features assigned to CH₂ and CH₃ stretching vibrations of lipid acyl chains and PO₂ stretching vibrations of nucleic acids. To assess the potential of IR spectroscopy for the diagnosis of breast tumor tissues, thin sections of tissue were mapped by FTIR microspectroscopy. The spectra of these maps were analyzed using functional group mapping techniques and cluster analysis, and the output values of the different approaches were then reassembled into IR images of the tissue. A comparison of the IR images with the standard light microscopic images of the corresponding areas suggested that: (i) chemical mapping based on single band intensities is an easy way to detect microscopic fat droplets within tissue; (ii) the comparison of IR images based on band intensities at 1054 and 1339 cm^-1 provides information on tissue areas containing tumor cells; (iii) cluster analysis of the spectra is superior to the single band approach and more appropriate for differentiation between tissue types.

Citation Download Citation

Heinz Fabian, Ralf Wessel, Michael Jackson, Arnfried Schwartz, Peter Lasch, Iduna Fichtner, Henry H. Mantsch, and Dieter Naumann "IR spectroscopy and IR microscopy of human breast tumors, xenografted breast tumors, and breast tumor cell lines", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306082

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available