Novel methods for fluorescence sensing

Joseph R. Lakowicz; Ignacy Gryczynski; Zygmunt Gryczynski; Jonathan D. Dattelbaum; Leah Tolosa; Govind Rao

doi:10.1117/12.347525

3 May 1999 Novel methods for fluorescence sensing

Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan D. Dattelbaum, Leah Tolosa, Govind Rao

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347525
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We describe a new method for fluorescence sensing based on measurements of the steady state polarization of an analyte- sensitive fluorophore in the presence of a reference fluorophore with known polarization. The basic concept is that the polarization of a mixture reflects a weighted average of the polarization of the emitting species. By use of reference fluorophores the starting values can be near zero, or near 0.9 for oriented films which contain the reference fluorophore. Changing intensities of the sensing fluorophore due to the analyte result in changes in the polarization of the combined emission. A wide dynamic range is available because of the freedom to select high or low starting polarization values. Polarization-based sensing was demonstrated for pH using 6- carboxy fluorescein. We also show that polarization sensing can be used for measurements of oxygen and glucose. Polarization sensing can have numerous applications in clinical and analytical chemistry.

Citation Download Citation

Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan D. Dattelbaum, Leah Tolosa, and Govind Rao "Novel methods for fluorescence sensing", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347525

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