Magnetic tweezers microscope for cellular manipulation

Chen-Yuan Dong; Hayden Huang; Jason D. B. Sutin; Hyuk-Sang Kwon; George E. Cragg; R. Gilbert; Richard T. Lee; Enrico Gratton; Roger D. Kamm; Douglas A. Lauffenburger; Peter T. C. So

doi:10.1117/12.384210

27 April 2000 Magnetic tweezers microscope for cellular manipulation

Chen-Yuan Dong, Hayden Huang, Jason D. B. Sutin, Hyuk-Sang Kwon, George E. Cragg, R. Gilbert, Richard T. Lee, Enrico Gratton, Roger D. Kamm, Douglas A. Lauffenburger, Peter T. C. So

Author Affiliations +

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384210
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We present the design of a magnetic tweezers microscope for cellular manipulation. Our design allows versatile and significant 3D stress application over a large sample region. For linear force application, forces up to 250 pN per 4.5 micrometers magnetic bead can be applied. Finite element analysis shows that variance in force level is around 10 percent within an area of 300 X 300 micrometers ². Our eight-pole design potentially allows 3D liner force application and exertion of torsional stress. Furthermore, our design allows high resolution imaging using high numerical aperture objective. Both finite element analysis of magnetic field distribution and force calibration of our design are presented. As a feasibility study, we incubated fibronectin coated 4.5 micrometers polystyrene beads with Swiss 3T3 mouse fibroblast cells. Under application around 250 pN of force per magnetic particle, we observed relative movement between attached magnetic and polystyrene beads to be on the order of 1 micrometers . Elastic, viscoelastic, and creeping responses of cell surfaces were observed. Our results are consistent with previous observations using similar magnetic techniques.

Citation Download Citation

Chen-Yuan Dong, Hayden Huang, Jason D. B. Sutin, Hyuk-Sang Kwon, George E. Cragg, R. Gilbert, Richard T. Lee, Enrico Gratton, Roger D. Kamm, Douglas A. Lauffenburger, and Peter T. C. So "Magnetic tweezers microscope for cellular manipulation", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384210

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Magnetism

Particles

Microscopes

Optical spheres

Calibration

Finite element methods

Magnetic tracking

Show All Keywords

Keywords/Phrases

Search In:

Publication Years