Scanning near-field optical microscopy of cell surfaces after structure conserving air drying

Birgit Perner; Michael Hausmann; Leo Wollweber; Alexander Rapp; Shamci Monajembashi; Karl-Otto Greulich

doi:10.1117/12.410639

18 December 2000 Scanning near-field optical microscopy of cell surfaces after structure conserving air drying

Birgit Perner, Michael Hausmann, Leo Wollweber, Alexander Rapp, Shamci Monajembashi, Karl-Otto Greulich

Proceedings Volume 4164, Laser Microscopy; (2000) https://doi.org/10.1117/12.410639
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Scanning near-field optical microscopy (SNOM) can simultaneously map topographic and optical properties of surfaces with a spatial resolution between that of far-field light microscopy and electron microscopy, i.e. in the range of 100 nm. Since commercially available SNOM instruments came on the market, this technique has become interesting for the routine biological research laboratory especially in combination with far-field light imaging. However, due to the usually applied shear-force feedback controlling the SNOM tip, this technique still poses several challenges for biological applications. In our experiments for instance imaging of soft samples, large topographical changes on structurally conserved cell surfaces, and in particular the requirement for completely dried specimen had to be considered. To visualize surfaces of cells fixed on standard glass slides by SNOM, an easy to handle, optimized protocol using dehydration and hexamethyldisilazane exposure before air drying was developed. Using the commercially available instrument SNOM 210 with micro-fabricated silicon nitride tips, it was shown for several cell systems that the cellular morphology and surface structures were well preserved after this procedure of drying.

Citation Download Citation

Birgit Perner, Michael Hausmann, Leo Wollweber, Alexander Rapp, Shamci Monajembashi, and Karl-Otto Greulich "Scanning near-field optical microscopy of cell surfaces after structure conserving air drying", Proc. SPIE 4164, Laser Microscopy, (18 December 2000); https://doi.org/10.1117/12.410639

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available