Online visualization of axial intensity distribution in spatially modulated illumination microscopy

Benno Albrecht; Rainer Heintzmann; Christoph G. Cremer

doi:10.1117/12.426781

10 May 2001 Online visualization of axial intensity distribution in spatially modulated illumination microscopy

Benno Albrecht, Rainer Heintzmann, Christoph G. Cremer

Proceedings Volume 4260, Optical Diagnostics of Living Cells IV; (2001) https://doi.org/10.1117/12.426781
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Recently, several light microscopical approaches, such as point spread function (PSF) engineering, Spectral Precision Distance Microscopy (SPDM) and related methods have demonstrated the feasibility of measurements considerably beyond the conventional Abbe-Limit of optical resolution in far field light microscopy. The Heidelberg Spatially Modulated Illumination (SMI) microscope is based on the generation of a standing wave light field in the direction of the optical axis of the system. The theoretical PSF is obtained by the manipulation of the sin²-shaped illumination PSF and the epifluorescent detection PSF. Here, we report a software method to obtain online visualization of the axial light distribution of any object detected, developed using Microsoft Visual C++ and based on Windows NT. This strongly facilitates routine application of SMI Microscopy.

Citation Download Citation

Benno Albrecht, Rainer Heintzmann, and Christoph G. Cremer "Online visualization of axial intensity distribution in spatially modulated illumination microscopy", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); https://doi.org/10.1117/12.426781

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